Difference between revisions of "Workflow"

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A '''workflow''' in bioinformatics can be defined as a series of computational or data manipulation steps. To compose and execute such sequences a variety of special [[wikipedia:Bioinformatics workflow management system|workflow management systems]] has been developed. All such systems are based on an abstract representation of how a computation proceeds in the form of a directed [[wikipedia:Graph (mathematics)#Directed graph|graph]], where each [[wikipedia:Vertex (graph theory)|node]] represents a task to be executed and edges represent either data flow or execution dependencies between different tasks. Each system typically provides visual front-end allowing the user to build and modify complex applications with little or no programming expertise.
  
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A '''workflow''' in bioinformatics can be defined as a series of computational or data manipulation steps. To compose and execute such sequences a variety of special [http://en.wikipedia.org/wiki/Bioinformatics_workflow_management_system workflow management systems] has been developed. All such systems are based on an abstract representation of how a computation proceeds in the form of a directed [http://en.wikipedia.org/wiki/Graph_(mathematics)#Directed_graph graph], where each [http://en.wikipedia.org/wiki/Vertex_(graph_theory) node] represents a task to be executed and edges represent either data flow or execution dependencies between different tasks. Each system typically provides visual front-end allowing the user to build and modify complex applications with little or no programming expertise.
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[[BioUML]] provides a workflow management system, which is intuitively handled through a simple drag-and-drop interface. With BioUML-related products users can either run pre-defined workflows or create their own for specific analysis purposes.
  
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BioUML provides a workflow management system, which is intuitively handled through a simple drag-and-drop interface. With BioUML-related products users can either run pre-defined workflows or create their own for specific analysis purposes.
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In BioUML the workflow is a special {{Type link|diagram}} type, so you can edit it using the [[diagram document]].
  
 
==Pre-defined workflows==
 
==Pre-defined workflows==
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Now, let's consider the following step-by-step example of creating a simple workflow for filtering table data.
 
Now, let's consider the following step-by-step example of creating a simple workflow for filtering table data.
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===Six steps to compose a simple workflow===
 
===Six steps to compose a simple workflow===
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[[File:Editing workflow diagram adding method.png|thumb|left|Composing a workflow diagram - Step 1: adding an analysis method (alternative ways shown in red and blue)]]
 
[[File:Editing workflow diagram adding method.png|thumb|left|Composing a workflow diagram - Step 1: adding an analysis method (alternative ways shown in red and blue)]]
 
Suppose you have specified the name and directory for your new workflow and its tab is active in the workspace,
 
Suppose you have specified the name and directory for your new workflow and its tab is active in the workspace,
 
[[File:Editing workflow diagram adding input table.png|thumb|Composing a workflow diagram - Step 2: adding an input table]]  
 
[[File:Editing workflow diagram adding input table.png|thumb|Composing a workflow diagram - Step 2: adding an input table]]  
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'''the first step''' is to '''add the analysis function''' to the workflow diagram. You can either drag and drop the '''Filter table''' method from the subdirectory '''Methods/Data''' in the '''Analyses''' tab of the navigation pane, or add the analysis-method item from the toolbox and choose '''Data/Filter table''' from the drop-down list of the '''Create new node''' dialog box. The item will appear on the diagram as a light blue rectangle labelled "Filter table".
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'''the first step''' is to '''add the analysis function''' to the workflow diagram. You can either drag and drop the <span class="plainlinks">[http://wiki.biouml.org/index.php/Filter_table_(analysis) '''Filter table''']</span> method from the subdirectory '''Methods/Data''' in the '''Analyses''' tab of the navigation pane, or add the analysis-method item from the toolbox and choose '''Data/Filter table''' from the drop-down list of the '''Create new node''' dialog box. The item will appear on the diagram as a light blue rectangle labelled "Filter table".
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'''Step 2.''' For '''creating the input table''' click on the green element in the tool bar, locate the cursor in the Work Space where you would like to put this element and  
 
'''Step 2.''' For '''creating the input table''' click on the green element in the tool bar, locate the cursor in the Work Space where you would like to put this element and  
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[[File:Editing workflow diagram adding edge.png|thumb|left|Composing a workflow diagram - Step 3: adding a directed edge]]
 
[[File:Editing workflow diagram adding edge.png|thumb|left|Composing a workflow diagram - Step 3: adding a directed edge]]
  
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'''Step 3.''' '''Joining elements''' on the diagram is done by clicking on the arrow symbol in the toolbar - a new window '''Create new edge''' will pop up. By clicking on the '''Table''' element in the workspace you select it as the '''Input node''' for this edge. Similarly, you click on the left table symbol in the '''Filter table''' element to select it as the '''Output node''' of this new edge. After pressing '''OK''' a new connection (edge) appears.
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[[File:Editing workflow diagram adding output table.png|thumb|Composing a workflow diagram - Step 3: adding an output table (A) and joining it to the method element (B)]]
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'''Step 3.''' '''Joining elements''' on the diagram is done by clicking on the arrow symbol in the toolbar - a new window '''Create new edge''' will pop up. By clicking on the '''Table''' element in the workspace you select it as the '''Input node''' for this edge. Similarly, you click on the input path (port) in the '''Filter table''' element to select it as the '''Output node''' of this new edge. After pressing '''OK''' a new connection (edge) appears.
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[[File:Editing workflow diagram adding output table.png|thumb|Composing a workflow diagram - Step 4: adding an output table (A) and joining it to the method element (B)]]
  
  
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As a result, we have now created one step of the workflow.  
 
As a result, we have now created one step of the workflow.  
  
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'''Step 5.''' To filter data, we have to '''define a filtering condition'''. To do this we have to create a new element '''Filtering condition''' (yellow element in the tool bar), which will be now of simple '''String''' type and which contains a filtering condition “Score > 2” in the '''Expression''' field. A new element '''Filtering condition''' is created. This element should now be connected to the analysis function “Filter table” in order to define the filtering condition that is going to be applied at this step of the workflow. To do that, click first on the '''Filter table''' element and open the parameters of this element in the operations field. After that, click on the field “Filtering condition” (1) in the parameter list and select it (a blue background color indicates that the field is selected). Click on the '''Bind property to variable''' button (2) in the toolbar of the Operations Field. And after that, move the cursor to the workspace and click on the '''Filtering condition''' element on the diagram (3). 
 
  
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So, the filtering condition parameter is now connected to the corresponding field of the '''Filter table''' function.
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'''Step 5.''' To filter data, it's necessary to '''define a filtering condition'''. For that create a new element '''Filtering condition''' (yellow element in the tool bar), which will be now of simple '''string''' type and which contains a filtering condition “Score > 2” in the '''Expression''' field. A new element '''Filtering condition''' is created.
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[[File:Editing workflow diagram adding filtering condition.png|thumb|left|Composing a workflow diagram - Step 5: adding a method condition (A) and joining it to the method element (B)]]
  
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'''Step 6.''' The workflow is now ready to be executed. To '''start the workflow''' please click on the '''Run workflow''' button in the toolbar of the operations field.   
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In the pop-up menu “Workflow parameters” you should specify the input table. Navigate to the folder with your tables and select a table which has a column “Score” and press OK. The workflow will be executed and a new table with a new name and the appendix “filtered” will be created in the same folder as the input table.
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This element should now be connected to the analysis function '''Filter table''' in order to define the filtering condition that is going to be applied at this step of the workflow. To do that, click first on the '''Filter table''' element and open the parameters of this element in the operations field (the '''Workflow''' tab). Then, click on the field '''Filtering expression''' on the parameter list to select it (the blue background color indicates that the field is selected). Click on the '''Bind property to variable''' icon in the toolbar of the operations field. And after that, move the cursor to the workspace and click on the '''Filtering condition''' element on the diagram - the filtering condition parameter gets now connected to the corresponding field of the '''Filter table''' function. 
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'''Step 6.''' The workflow is now ready to be executed. To '''start the workflow''' please click on the '''Run workflow''' button ([[File:Run workflow icon.png|caption]]) in the toolbar of the operations field.   
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In the pop-up menu '''Workflow parameters''' you are to specify the input table. Navigate to the folder with your tables and select a table which has a column “Score” and press OK. The workflow will be executed and a new table with a new name and the appendix “filtered” will be created in the same folder as the input table.
  
 
===Complex workflows===
 
===Complex workflows===
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{{draft}}
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[[File:Editing workflow diagram more complex.png|thumb|An example workflow diagram with additional steps]]
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More complex workflows are created by adding next workflow steps and by connecting them through a common data element. As shown in the figure, the output element '''Table filtered''' of the first step is used as the input element of the second step of the workflow <span class="plainlinks">[http://wiki.biouml.org/index.php/Regulator_search_(analysis) '''Regulator search''']</span>. You can also see that a new input parameter '''Species''' has been added, which appears now among the workflow parameters upon starting the workflow. With this, you can select a taxonomic species (presently human, mouse or rat) for the table you are going to run through the workflow.
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[[File:research diagram.png|thumb|Example of a research diagram]]
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<div id="research diagram"></div>
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'''Note:''' During execution of a workflow a “research diagram” is saved (you can specify the name of this diagram before starting of the workflow).
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The research diagram contains the history of the workflow execution with the names of all input and output files. It also contains all the links to these tables, so that you can easily open them by clicking on the respective element in the diagram.
  
 
===Cycles and scripts===
 
===Cycles and scripts===
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{{draft}}
 
  
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Another element available for composing workflows is '''cycle'''. It can be created using the '''cycle''' button in the toolbox. In the '''Create new node''' dialog box it’s necessary to specify the '''Name''' of the cycle (the “cycle variable”), and to choose the appropriate '''Type''' and '''Cycle type'''. 
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[[File:Editing workflow diagram cycle params.png|thumb|Setting parameters of a cycle]]
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The option “All elements in collection” ('''Cycle type''') together with the '''Type''' “Data element” and some folder name in the field '''Expression''' means that all data elements from that folder will be taken one by one as cycle variable values. For example, when selecting '''Cycle type''' “Table columns”, '''Expression''' should specify the name of this table. Or when choosing '''Cycle type''' “Range (from..to)” for '''Type''' “Integer number” and '''Expression''' as “2..6”, the cycle will be executed by assigning the values 2,3,4,5 and 6 to the cycle variable.
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[[File:Editing workflow diagram script params.png|thumb|left|The dialog box of a new script]]
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The '''script''' workflow element represents a code written in [[wikipedia:JavaScript|JavaScript]], which can be executed during the workflow run. To add a script user should press the '''analysis-script''' toolbox icon, click on the proper place in the workflow diagram area and type JavaScript code in the '''Script source''' field.
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All variables defined in a workflow (green and orange boxes) are available inside the script as JavaScript variables. If the name doesn’t contain spaces, it can be used as it is, the name should be put into <code>$["…"]</code> otherwise. For example, the variable “TableColumn” can be accessed from a script either by name <code>TableColumn</code> or by <code>$["TableColumn"]</code>, but “Table column N1” should be accessed as <code>$["Table column N1"]</code>.
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It's also possible to change the workflow variable from the script. To achieve this you should perform the following steps:
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# Create an arrow which starts from the script workflow element and ends in variable node you want to change. Only variables connected with such arrows can be written.
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# Use <code>$["&lt;VariableName&gt;"] = &lt;new_value&gt;</code> in the script. Implicit type conversion may be performed if necessary.
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''Example 1. Print column names''
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[[File:Editing workflow diagram with a cycle.png|thumb|A workflow diagram with a cycle to print table column names]]
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In this example the data element parameter '''InputTable''' (shown as a green box) is first added to the workflow. Then a cycle with the following settings is added:
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* Name: TableColumn
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* Type: String
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* Cycle type: Table columns
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* Expression: $InputTable$.
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Note that expression is set to $InputTable$ for a cycle.
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Then a script element with following code will be created inside a cycle:
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<pre>print(TableColumn);</pre>
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When the '''Run''' button is pressed, the workflow will ask for a table path, and then will print all column names into the workflow output log.
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''Example 2. Run [[wikipedia:Gene Ontology|GO]] classification for all tables in a folder''
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This workflow (see the figure) contains three input elements: '''InputFolder''', where one or more Tables should be placed; '''ResultFolder''' that will be created by the method <span class="plainlinks">[http://wiki.biouml.org/index.php/Create_folder_(analysis) '''Create folder''']</span>, and the data element '''Species''', required for table conversion and functional classification.
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[[File:Editing workflow diagram GO classification.png|thumb|A workflow diagram to GO classify all tables in a folder]]
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The cycle has following settings:
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* Name: Table
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* Type: Data element
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* Cycle type: All elements in collection
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* Expression: $InputFolder$
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Here, the cycle variable named '''Table''' will adopt the names of the tables in the InputFolder. Then it goes to the method <span class="plainlinks">[http://wiki.biouml.org/index.php/Convert_table_(analysis) '''Convert table''']</span>, and identifiers are converted to Ensembl genes, according to the analysis settings below:
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* Input type: (Auto)
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* Output type: Genes:Ensemble
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* Species: Human (Homo sapiens)
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* Name of main column: (no column)
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* Aggregator for numbers: average
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The conversion result is taken as the input set for the <span class="plainlinks">[http://wiki.biouml.org/index.php/Functional_classification_(analysis) '''Functional classification''']</span> analysis:
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* Source data set: <same as the output table at the previous step>
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* Species: Human (Homo sapiens)
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* Classification: Full gene ontology classification
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* Minimal hits to group: 2
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* Only over-represented: V (yes)
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* P-value threshold: 0.05
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The output of the functional classification is a data element named  '''GOres''' with '''Expression''' $ResultsFolder$/$Table/name$ GO.
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When the '''Run''' button is pressed, the workflow will start.
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== See also ==
 
== See also ==
 
* [[:Category:Workflows|List of workflows]]
 
* [[:Category:Workflows|List of workflows]]
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[[Category:Diagram types]]

Latest revision as of 16:54, 3 December 2013

A workflow in bioinformatics can be defined as a series of computational or data manipulation steps. To compose and execute such sequences a variety of special workflow management systems has been developed. All such systems are based on an abstract representation of how a computation proceeds in the form of a directed graph, where each node represents a task to be executed and edges represent either data flow or execution dependencies between different tasks. Each system typically provides visual front-end allowing the user to build and modify complex applications with little or no programming expertise.

BioUML provides a workflow management system, which is intuitively handled through a simple drag-and-drop interface. With BioUML-related products users can either run pre-defined workflows or create their own for specific analysis purposes.

In BioUML the workflow is a special Type-diagram-icon.png diagram type, so you can edit it using the diagram document.

Contents

[edit] Pre-defined workflows

BioUML, and especially the geneXplain platform, facilitates standard analyses through a number of pre-composed workflows concatenating some of the most important modules, which also allows users to start with their first analyses right away, even before having learned all sophisticated details of the platform.

With any workflow the following steps are normally taken:

  • importing data into the project (if necessary),
  • data normalization (if necessary),
  • selecting the appropriate workflow,
  • specifying the input file(s),
  • parameters setting,
  • specifying the output directory,
  • running the workflow,
  • viewing the output.

A few pre-defined workflows in BioUML workbench are available as example data (e.g. Data tab in navigation pane > data > Examples > ChIPMunk workflows). GeneXplain offers a somewhat richer choice of ready-made workflows (see the list of workflows below) with all the links launching them right from the start page.


[edit] Creating your own workflows

To create a new workflow in BioUML workbench you first need to go to the Data folder (or a subfolder within) of your project in which the new workflow will be stored (e.g. Data tab in navigation pane > data > Collaboration > test project > Data folder). As soon as you click on the Data folder in the navigation pane, a number of operation icons appears in the navigation toolbar. The names of the operations are shown as tooltip text (just place the mouse pointer over an icon). Click on the New workflow option.

Creating a new workflow file

To create a workflow in geneXplain go to the Start page and click Create your own workflow under the list of pre-defined workflow groups.

At this point you will be asked to specify the name of the new workflow in the pop-up dialog box. Here you can also choose a different location to save the workflow by navigating the directories tree and creating (sub)folders.

As you press Ok in the dialog box, a new tab opens in the workspace, where you can design a new workflow diagram. The workflow diagram represents different analysis functions being connected by input and output files. The resulting directed graph visualizes the sequence of analysis steps in the workflow. The diagram also may contain parameters, which are to be defined by the user.


You can add nodes to the graph by dragging and dropping items directly from the Analyses tab of the navigation pane (Analyses tab > analyses > Methods > ...) or by using the toolbox within the tab. The toolbox contains icons for such types of nodes as analysis methods, analysis parameters, analysis expressions, cycles and analysis scripts as well as for the Select tool, directed edges, notes and note edges. To add a node from the toolbox click first on the icon, then within the workspace, set parameters in the Create new node dialog box and press Ok - the node will appear in the graph. To add an edge just click on the icon in the toolbox, specify the output and input nodes in the Create new edge dialog box and press Ok.

Upon clicking on any component of the workflow you can see the information about this particular element in the operations field below.

Now, let's consider the following step-by-step example of creating a simple workflow for filtering table data.


[edit] Six steps to compose a simple workflow

Composing a workflow diagram - Step 1: adding an analysis method (alternative ways shown in red and blue)

Suppose you have specified the name and directory for your new workflow and its tab is active in the workspace,

Composing a workflow diagram - Step 2: adding an input table

the first step is to add the analysis function to the workflow diagram. You can either drag and drop the Filter table method from the subdirectory Methods/Data in the Analyses tab of the navigation pane, or add the analysis-method item from the toolbox and choose Data/Filter table from the drop-down list of the Create new node dialog box. The item will appear on the diagram as a light blue rectangle labelled "Filter table".


Step 2. For creating the input table click on the green element in the tool bar, locate the cursor in the Work Space where you would like to put this element and click. A new window Create new node will pop up, where you are to define the parameters of the element:

  • Name field: the title of the element;
  • Type: select “Data element”, for any objects like tables.
  • In the field Default value you can type a full folder path where the table is located. You can also use some global variables, like “$project$” that already contain the full path (by clicking on the “…” button you can access all the global variables defined for this workflow.
  • Rank (sort order): this number gives the position of this input element in the list of all elements upon starting the workflow.
  • Role: "Input", since we are using this element for inputting a table into the workflow.

The selected item will appear on the diagram as a light green arrow-like pentagon labelled according to the Name parameter.

Composing a workflow diagram - Step 3: adding a directed edge


Step 3. Joining elements on the diagram is done by clicking on the arrow symbol in the toolbar - a new window Create new edge will pop up. By clicking on the Table element in the workspace you select it as the Input node for this edge. Similarly, you click on the input path (port) in the Filter table element to select it as the Output node of this new edge. After pressing OK a new connection (edge) appears.

Composing a workflow diagram - Step 4: adding an output table (A) and joining it to the method element (B)


Step 4. The same way you can create now an output table element on the diagram by selecting the yellow element in the tool bar (since it is going to be an intermediate table for further use in the next steps of the workflow) and connecting it with the output icon of the Filter table element. In the Expression field you can now use a new global variable “$Table$” which will contain, during the run of the workflow, the name of the table which you have entered. So in this case we are creating a new name for the future output table “$Table$ filtered” by adding to the name of the input table an ending “filtered”. As a result, we have now created one step of the workflow.


Step 5. To filter data, it's necessary to define a filtering condition. For that create a new element Filtering condition (yellow element in the tool bar), which will be now of simple string type and which contains a filtering condition “Score > 2” in the Expression field. A new element Filtering condition is created.

Composing a workflow diagram - Step 5: adding a method condition (A) and joining it to the method element (B)


This element should now be connected to the analysis function Filter table in order to define the filtering condition that is going to be applied at this step of the workflow. To do that, click first on the Filter table element and open the parameters of this element in the operations field (the Workflow tab). Then, click on the field Filtering expression on the parameter list to select it (the blue background color indicates that the field is selected). Click on the Bind property to variable icon in the toolbar of the operations field. And after that, move the cursor to the workspace and click on the Filtering condition element on the diagram - the filtering condition parameter gets now connected to the corresponding field of the Filter table function.


Step 6. The workflow is now ready to be executed. To start the workflow please click on the Run workflow button (caption) in the toolbar of the operations field. In the pop-up menu Workflow parameters you are to specify the input table. Navigate to the folder with your tables and select a table which has a column “Score” and press OK. The workflow will be executed and a new table with a new name and the appendix “filtered” will be created in the same folder as the input table.

[edit] Complex workflows

An example workflow diagram with additional steps

More complex workflows are created by adding next workflow steps and by connecting them through a common data element. As shown in the figure, the output element Table filtered of the first step is used as the input element of the second step of the workflow Regulator search. You can also see that a new input parameter Species has been added, which appears now among the workflow parameters upon starting the workflow. With this, you can select a taxonomic species (presently human, mouse or rat) for the table you are going to run through the workflow.

Example of a research diagram


Note: During execution of a workflow a “research diagram” is saved (you can specify the name of this diagram before starting of the workflow).

The research diagram contains the history of the workflow execution with the names of all input and output files. It also contains all the links to these tables, so that you can easily open them by clicking on the respective element in the diagram.

[edit] Cycles and scripts

Another element available for composing workflows is cycle. It can be created using the cycle button in the toolbox. In the Create new node dialog box it’s necessary to specify the Name of the cycle (the “cycle variable”), and to choose the appropriate Type and Cycle type.

Setting parameters of a cycle

The option “All elements in collection” (Cycle type) together with the Type “Data element” and some folder name in the field Expression means that all data elements from that folder will be taken one by one as cycle variable values. For example, when selecting Cycle type “Table columns”, Expression should specify the name of this table. Or when choosing Cycle type “Range (from..to)” for Type “Integer number” and Expression as “2..6”, the cycle will be executed by assigning the values 2,3,4,5 and 6 to the cycle variable.

The dialog box of a new script

The script workflow element represents a code written in JavaScript, which can be executed during the workflow run. To add a script user should press the analysis-script toolbox icon, click on the proper place in the workflow diagram area and type JavaScript code in the Script source field.

All variables defined in a workflow (green and orange boxes) are available inside the script as JavaScript variables. If the name doesn’t contain spaces, it can be used as it is, the name should be put into $["…"] otherwise. For example, the variable “TableColumn” can be accessed from a script either by name TableColumn or by $["TableColumn"], but “Table column N1” should be accessed as $["Table column N1"].

It's also possible to change the workflow variable from the script. To achieve this you should perform the following steps:

  1. Create an arrow which starts from the script workflow element and ends in variable node you want to change. Only variables connected with such arrows can be written.
  2. Use $["<VariableName>"] = <new_value> in the script. Implicit type conversion may be performed if necessary.

Example 1. Print column names

A workflow diagram with a cycle to print table column names

In this example the data element parameter InputTable (shown as a green box) is first added to the workflow. Then a cycle with the following settings is added:

  • Name: TableColumn
  • Type: String
  • Cycle type: Table columns
  • Expression: $InputTable$.

Note that expression is set to $InputTable$ for a cycle.

Then a script element with following code will be created inside a cycle: 

print(TableColumn);

When the Run button is pressed, the workflow will ask for a table path, and then will print all column names into the workflow output log.


Example 2. Run GO classification for all tables in a folder

This workflow (see the figure) contains three input elements: InputFolder, where one or more Tables should be placed; ResultFolder that will be created by the method Create folder, and the data element Species, required for table conversion and functional classification.

A workflow diagram to GO classify all tables in a folder

The cycle has following settings:

  • Name: Table
  • Type: Data element
  • Cycle type: All elements in collection
  • Expression: $InputFolder$

Here, the cycle variable named Table will adopt the names of the tables in the InputFolder. Then it goes to the method Convert table, and identifiers are converted to Ensembl genes, according to the analysis settings below:

  • Input type: (Auto)
  • Output type: Genes:Ensemble
  • Species: Human (Homo sapiens)
  • Name of main column: (no column)
  • Aggregator for numbers: average


The conversion result is taken as the input set for the Functional classification analysis:

  • Source data set: <same as the output table at the previous step>
  • Species: Human (Homo sapiens)
  • Classification: Full gene ontology classification
  • Minimal hits to group: 2
  • Only over-represented: V (yes)
  • P-value threshold: 0.05

The output of the functional classification is a data element named GOres with Expression $ResultsFolder$/$Table/name$ GO.

When the Run button is pressed, the workflow will start.

 

[edit] See also

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