Difference between revisions of "Gene set enrichment analysis - select a classification (Gene table) (workflow)"

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[[File:Gene-set-enrichment-analysis-select-a-classification-Gene-table-workflow-overview.png|400px]]
 
[[File:Gene-set-enrichment-analysis-select-a-classification-Gene-table-workflow-overview.png|400px]]
 
== Description ==
 
== Description ==
This workflow is designed to perform Gene Set Enrichment Analysis, GSEA, as it is described at [http://www.broadinstitute.org/gsea/index.jsp http://www.broadinstitute.org/gsea/index.jsp]. As input, any gene or protein table that includes fold change (or any other numerical value that can be used as a weighted column) can be taken.
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This workflow performs ''Gene Set Enrichment Analysis'' for the input table of genes or proteins. It is designed to enable a comfortable selection of several input parameters in the input form, especially selection of an ontology.  Any ontology available with a given subscription can be chosen from the drop-down menu in the field ''Classification''.
  
 At the first step, the input table is converted into a table with Ensembl Gene IDs.
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The Input table in the first step is converted to Ensembl Gene IDs, and in the next step an Ensembl gene table is submitted to ''Enrichment analysis'' using the ontology selected. 
  
This table with Ensembl Gene IDs is subjected to GSEA. Enrichment analysis is done in parallel by the following ontologies: GO biological processes, GO cellular components, GO molecular functions and by the Reactome pathways.
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The output contains the results of the enrichment analysis. For each ontological term several parameters are calculated, including nominal p-value, ES, NES, Rank at max, FDR, as well as hit names, the link to the corresponding ontological term, and the link to open a visualization plot.
 
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For each ontological term several parameters are calculated, including nominal p-value, ES, NES, as well as hit names, the link to the corresponding ontological term, and the link to open a visualization plot.
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== Parameters ==
 
== Parameters ==

Revision as of 11:49, 30 July 2013

Workflow title
Gene set enrichment analysis - select a classification (Gene table)
Provider
geneXplain GmbH

Workflow overview

Gene-set-enrichment-analysis-select-a-classification-Gene-table-workflow-overview.png

Description

This workflow performs Gene Set Enrichment Analysis for the input table of genes or proteins. It is designed to enable a comfortable selection of several input parameters in the input form, especially selection of an ontology.  Any ontology available with a given subscription can be chosen from the drop-down menu in the field Classification.

The Input table in the first step is converted to Ensembl Gene IDs, and in the next step an Ensembl gene table is submitted to Enrichment analysis using the ontology selected. 

The output contains the results of the enrichment analysis. For each ontological term several parameters are calculated, including nominal p-value, ES, NES, Rank at max, FDR, as well as hit names, the link to the corresponding ontological term, and the link to open a visualization plot.

Parameters

Input table
Enrichment weight column
Enter the name of the column from the Input table with numerical values
Species
Classification
Min number of hits to group
Results table
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