Meta analysis

Meta-analysis

The aim of a meta-analysis is to identify up- and down-regulated genes from different independent microarray experiments. Such a meta-analysis is based on the hypergeometric analysis method. Within the framework of a meta-analysis, a hypergeometric analysis is applied to sets of gene profiles. The obtained results are the initial data for a subsequent meta-analysis. It uses the same procedure as a hypergeometric analysis for testing the hypothesis that the that score obtained in previous tests deviates from 0 (Boundary Value = 0) just by chance. The result is a table with meta scores that indicate.

Parameters:

• Tables - tables from the input data collection to be used for the meta analysis

  Note: for proper work tables should have only one column with scores obtained by Hypergeometric analysis (without fold change, details or annotations).

• Output type - the type of genes to be included in result:
  • Up- and down-regulated
  • Up-regulated
  • Down-regulated
• P-value threshold - threshold for P-value (only elements with lower P-value will be included in the results).
• Calculate FDR - the test method for calculation of False Discovery Rate (FDR) - an average rate of mistakenly found up- or down-regulated genes under the given P-value threshold. It randomly permutates the data 50 times and applies the selected meta-analysis to each randomized table. FDR is calculated separately for up- and down-regulated genes according to the formula:

  

• Output table - the path in the BioUML repository where the result table will be stored. If a table with the specified path already exists it will be replaced.