Difference between revisions of "Ribo-Seq and mRNA features forming (analysis)"

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==== Parameters: ====
 
==== Parameters: ====
  
* '''Ribo-Seq data set name''' – Select Ribo-Seq data set
+
* '''Data set name''' – Select data set
* '''Path to folder with data sets''' – Path to folder with data sets
+
 
* '''Sequences collection''' – Select a source of nucleotide sequences
 
* '''Sequences collection''' – Select a source of nucleotide sequences
 
** '''Sequences source''' – Select database to get sequences from or <nowiki>'</nowiki>Custom<nowiki>'</nowiki> to specify sequences location manually
 
** '''Sequences source''' – Select database to get sequences from or <nowiki>'</nowiki>Custom<nowiki>'</nowiki> to specify sequences location manually
 
** '''Sequence collection''' – Specify path to folder containing sequences if <nowiki>'</nowiki>Custom<nowiki>'</nowiki> sequences source is selected
 
** '''Sequence collection''' – Specify path to folder containing sequences if <nowiki>'</nowiki>Custom<nowiki>'</nowiki> sequences source is selected
* '''Input track''' – Path to domestic track with Ribo-Seq dataset
+
* '''Two tables for calculation of translation (initiation) efficiency, TE (TIE)''' – Two tables for calculation of translation (initiation) efficiency, TE (TIE)
* '''Minimal reads number threshold''' – Peaks for which number of reads exeeds this threshold will be analized
+
** '''Path to table with mRNA-Seq data''' – Path to table with mRNA-Seq data
 +
** '''Column name with mRNA-Seq reads number''' – Select column name with mRNA-Seq reads number
 +
** '''mRNA-Seq reads threshold''' – Transcripts for which mRNA-Seq reads number are grater than this threshold will be considered only
 +
** '''Path to table with Ribo-Seq data''' – Path to table with Ribo-Seq data
 +
** '''Column name with Ribo-Seq reads number''' – Select column name with Ribo-Seq reads number
 +
** '''Ribo-Seq reads threshold''' – Transcripts for which Ribo-Seq reads number are grater than this threshold will be considered only
 +
** '''Column name with start codon positions''' – Select column name with start codon positions
 +
** '''Column name with transcript names''' – Column name with transcript names
 +
* '''Path to folder with special data sets''' – Path to folder with special data sets
 
* '''Start codon type''' – Start codon type
 
* '''Start codon type''' – Start codon type
 +
* '''Order of start codon''' – Order of start codon
 
* '''mRNA and Ribo-Seq features''' – Select mRNA and Ribo-Seq features
 
* '''mRNA and Ribo-Seq features''' – Select mRNA and Ribo-Seq features
 +
* '''Sequence sample''' – Sequence sample
 +
** '''Are mRNA fragments near start codons?''' – Are mRNA fragments near start codons (or near stop codons)?
 +
** '''Left boundary of each mRNA fragment''' – Left boundary of each mRNA fragment with respect to start (or stop) codon
 +
** '''Right boundary of each mRNA fragment''' – Right boundary of each mRNA fragment with respect to start (or stop) codon
 +
* '''Paths to matrices''' – Paths to matrices
 
* '''Do exclude missing data?''' – Do exclude missing data? (If <nowiki>'</nowiki>no<nowiki>'</nowiki> then possible missing data will be included as NaN-values
 
* '''Do exclude missing data?''' – Do exclude missing data? (If <nowiki>'</nowiki>no<nowiki>'</nowiki> then possible missing data will be included as NaN-values
 
* '''Output table path''' – Path to the output table
 
* '''Output table path''' – Path to the output table

Latest revision as of 19:00, 13 February 2017

Analysis title
Default-analysis-icon.png Ribo-Seq and mRNA features forming
Provider
Institute of Systems Biology
Class
RiboSeqAndMrnaFeaturesForming
Plugin
biouml.plugins.bindingregions (Binding-regions related analyses)

[edit] Description

Create data matrix with Ribo-Seq and mRNA features and write it as TableDataCollection

[edit] Parameters:

  • Data set name – Select data set
  • Sequences collection – Select a source of nucleotide sequences
    • Sequences source – Select database to get sequences from or 'Custom' to specify sequences location manually
    • Sequence collection – Specify path to folder containing sequences if 'Custom' sequences source is selected
  • Two tables for calculation of translation (initiation) efficiency, TE (TIE) – Two tables for calculation of translation (initiation) efficiency, TE (TIE)
    • Path to table with mRNA-Seq data – Path to table with mRNA-Seq data
    • Column name with mRNA-Seq reads number – Select column name with mRNA-Seq reads number
    • mRNA-Seq reads threshold – Transcripts for which mRNA-Seq reads number are grater than this threshold will be considered only
    • Path to table with Ribo-Seq data – Path to table with Ribo-Seq data
    • Column name with Ribo-Seq reads number – Select column name with Ribo-Seq reads number
    • Ribo-Seq reads threshold – Transcripts for which Ribo-Seq reads number are grater than this threshold will be considered only
    • Column name with start codon positions – Select column name with start codon positions
    • Column name with transcript names – Column name with transcript names
  • Path to folder with special data sets – Path to folder with special data sets
  • Start codon type – Start codon type
  • Order of start codon – Order of start codon
  • mRNA and Ribo-Seq features – Select mRNA and Ribo-Seq features
  • Sequence sample – Sequence sample
    • Are mRNA fragments near start codons? – Are mRNA fragments near start codons (or near stop codons)?
    • Left boundary of each mRNA fragment – Left boundary of each mRNA fragment with respect to start (or stop) codon
    • Right boundary of each mRNA fragment – Right boundary of each mRNA fragment with respect to start (or stop) codon
  • Paths to matrices – Paths to matrices
  • Do exclude missing data? – Do exclude missing data? (If 'no' then possible missing data will be included as NaN-values
  • Output table path – Path to the output table
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