Difference between revisions of "Track statistics (analysis)"
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:[[Institute of Systems Biology]] | :[[Institute of Systems Biology]] | ||
Revision as of 19:00, 13 February 2017
- Analysis title
Track statistics
- Provider
- Institute of Systems Biology
- Class
SequenceStatistics- Plugin
- ru.biosoft.bsa (Bio-sequences analyses plugin)
Description
Gather various statistics about track or FASTQ file
Parameters:
- Source – Whether to get input data from track or from FASTQ
- Input track – Track to process
- FASTQ file – FASTQ file with reads to analyze
- Quality encoding – This specifies how phred quality values are encoded in the FASTQ file. In most of the cases system detects this value automatically. You may change it manually if auto-detection worked incorrectly.
- CSFasta file – File containing reads in color space
- Qual file – File containing corresponding quality values
- Alignment – Whether to align sites on left or right
- Processors – List of methods to gather the statistics
- Basic statistics – Gathers basic statistics like reads count and average read length
- Quality per base – Distribution of phred quality score along the bases
- Quality per sequence – Distribution of phred quality score among the sequences
- Nucleotide content per base – Distribution of individual nucleotides along the bases
- GC content per base – Distribution of GC along the bases
- GC content per sequence – Draws a distribution of GC content among reads
- N content per base – Distribution of 'N' along the bases
- Sequence length distribution – Calculates distribution of read lengths and outputs them as the table and as the chart
- Duplicate sequences – Calculate the rate of sequences duplication: how many sequences occurs 2, 3 and so on times relative to unique sequences. This statistic is based on the first 200000 reads
- Overrepresented sequences – Look for sequences which appear in more than 0.1% cases
- Overrepresented K-mers – Search for K-mers which are represented 3x times per sequence or 5x times per position
- Overrepresented prefixes – Search for read prefixes (starting from the read start) up to 15 bp long which are overrepresented in the set.
- Output path – Path to the output folder (will be created)
