Difference between revisions of "Analyze promoters (GTRD) (workflow)"
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At the next step, promoters are analyzed for potential cis-regulatory sites. Promoters in this workflow are defined as sequences from -1000 to +100 relative to the transcription start sites, as they are annotated in Ensembl. | At the next step, promoters are analyzed for potential cis-regulatory sites. Promoters in this workflow are defined as sequences from -1000 to +100 relative to the transcription start sites, as they are annotated in Ensembl. | ||
| − | Site search is done with the | + | Site search is done with the help of the TRANSFAC® library of the positional weight matrices, PWMs, namely with the profile vertebrate_non_redundant_minSUM. |
At the same step, frequencies of putative TFBSs are compared between Yes set and No set to identify sites overrepresented in Yes set versus No set. Default No set in the workflow is a set of housekeeping genes for the corresponding species (as they are published in PMID: 19534766) | At the same step, frequencies of putative TFBSs are compared between Yes set and No set to identify sites overrepresented in Yes set versus No set. Default No set in the workflow is a set of housekeeping genes for the corresponding species (as they are published in PMID: 19534766) | ||
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Next, the list of PWMs is converted into a table of transcription factors. Two tables are produced, with Ensembl Gene IDs and with Entrez IDs. | Next, the list of PWMs is converted into a table of transcription factors. Two tables are produced, with Ensembl Gene IDs and with Entrez IDs. | ||
| − | Finally, both tables with transcription factors are annotated with additional information, gene description and gene symbols. | + | Finally, both tables with transcription factors are annotated with additional information, gene description and gene symbols. |
| − | The | + | The output is a new folder with several tables, including a summary of the predicted TFBSs, genomic tracks of the Yes and No promoters and sites, as well as the tables with transcription factors potentially regulating the genes in the Yes set. |
| + | |||
| + | This workflow is available together with a valid TRANSFAC® license. | ||
== Parameters == | == Parameters == | ||
| − | ;Input gene set | + | ;Input gene set (Yes set) |
| + | ;Profile | ||
;Species | ;Species | ||
| + | ;No set | ||
| + | ;5<nowiki>'</nowiki> flank | ||
| + | :Position relative to TSS, bp | ||
| + | ;3<nowiki>'</nowiki> flank | ||
| + | :Position relative to TSS, bp | ||
;Results folder | ;Results folder | ||
Revision as of 11:29, 24 May 2013
- Workflow title
- Analyze promoters (GTRD)
- Provider
- geneXplain GmbH
Workflow overview
Description
This workflow is designed to search for putative transcription factor binding sites, TFBS, on the promoters of an input gene set.
As input, any gene or protein table can be submitted. The input table contains genes under study, and it is called “Yes” set.
At the first step, the input table is converted into a table with Ensembl Gene IDs.
At the next step, promoters are analyzed for potential cis-regulatory sites. Promoters in this workflow are defined as sequences from -1000 to +100 relative to the transcription start sites, as they are annotated in Ensembl.
Site search is done with the help of the TRANSFAC® library of the positional weight matrices, PWMs, namely with the profile vertebrate_non_redundant_minSUM.
At the same step, frequencies of putative TFBSs are compared between Yes set and No set to identify sites overrepresented in Yes set versus No set. Default No set in the workflow is a set of housekeeping genes for the corresponding species (as they are published in PMID: 19534766)
The result of this step is a list of PWMs the hits of which are overrepresented in Yes set versus No set.
Next, the list of PWMs is converted into a table of transcription factors. Two tables are produced, with Ensembl Gene IDs and with Entrez IDs.
Finally, both tables with transcription factors are annotated with additional information, gene description and gene symbols.
The output is a new folder with several tables, including a summary of the predicted TFBSs, genomic tracks of the Yes and No promoters and sites, as well as the tables with transcription factors potentially regulating the genes in the Yes set.
This workflow is available together with a valid TRANSFAC® license.
Parameters
- Input gene set (Yes set)
- Profile
- Species
- No set
- 5' flank
- Position relative to TSS, bp
- 3' flank
- Position relative to TSS, bp
- Results folder
