Difference between revisions of "Fastq mcf"
From BioUML platform
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fastq-mcf attempts to: | fastq-mcf attempts to: | ||
| − | * Detect & remove sequencing adapters and primers | + | *Detect & remove sequencing adapters and primers |
| − | * Detect limited skewing at the ends of reads and clip | + | *Detect limited skewing at the ends of reads and clip |
| − | * Detect poor quality at the ends of reads and clip | + | *Detect poor quality at the ends of reads and clip |
| − | * Detect Ns, and remove from ends | + | *Detect Ns, and remove from ends |
| − | * Remove reads with CASAVA 'Y' flag (purity filtering) | + | *Remove reads with CASAVA 'Y' flag (purity filtering) |
| − | * Discard sequences that are too short after all of the above | + | *Discard sequences that are too short after all of the above |
| − | * Keep multiple mate-reads in sync while doing all of the above | + | *Keep multiple mate-reads in sync while doing all of the above |
Revision as of 17:17, 25 March 2019
Introduction
fastq-mcf attempts to:
*Detect & remove sequencing adapters and primers *Detect limited skewing at the ends of reads and clip *Detect poor quality at the ends of reads and clip *Detect Ns, and remove from ends *Remove reads with CASAVA 'Y' flag (purity filtering) *Discard sequences that are too short after all of the above *Keep multiple mate-reads in sync while doing all of the above
